Environmental DNA Sampling and Experiment Manual
The eDNA Society publishes the standardized manual for eDNA analysis, titled “Environmental DNA Sampling and Experiment Manual.” We hope this manual will contribute to addressing global biodiversity loss and promoting conservation.
- Version 2.1 (Published on April 25, 2019)
If you use our manual in any publications, please include the following citation:
Minamoto T, Miya M, Sado T, Seino S, Doi H, Kondoh M, Nakamura K, Takahara T, Yamamoto S, Yamanaka H, Araki H, Iwasaki W, Kasai A, Masuda R, Uchii K (2021) An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols. Environmental DNA 3: 8-13 https://doi.org/10.1002/edn3.121
Supplemental videos on sampling procedures
Note that the videos are provided only in Japanese.- Water sampling using a bucket — 2.5 min
- Water sampling using a cup with a handle — 2.5 min
- Water sampling into a plastic bag — 6 min
- Filtration using a syringe — 3 min
- Addition of preservatives — 3 min
- Filtration using a caulking gun — 4 min
For the metabarcoding part, you may refer to the following video offered at CEARAC (Coastal Environmental Assessment Regional Activity Centre) youtube channel.
English version (approx. 50 min.)
